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. 2013 Feb 8;8(2):e55960. doi: 10.1371/journal.pone.0055960

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© 2013 Morita-Ishihara et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) GST-EspO1-2 binding assays were performed using cell lysates expressing Flag-tagged Map, EspM2, EspM2 or EspG. Proteins that bound to GST-EspO1-2 and GST-alone were analyzed by SDS-PAGE followed by immunoblotting with anti-Flag antibody. (B) To map the EspM2-binding site in EspO1-2, a series of truncated GST-EspO1-2 peptides was used in binding assays with cell lysate expressing Flag-tagged EspM2. (C) Co-localization of RFP-EspO1-2 and GFP-EspM2 in HeLa cells. HeLa cells were transfected with pRFP-EspO1-2, pRFP-EspO1-2 1–60aa or pRFP (red) and pEGFP-EspM2 (green).