|
Neisseria meningitidis
|
|
|
| M7 |
Unencapsulated NMB derivative |
[24]
|
| JC2 |
M7 mtrR::kana
|
This study |
| JC3 |
As JC2 but mtrR+ delivered using vector pGCC3 |
This study |
| JC4 |
As JC3 but mtrR+ delivered using vector pGCC4 |
This study |
| JC5 |
M7 farR::spcb
|
This study |
| JC6 |
As JC5 but farR+ delivered using vector pGCC3 |
This study |
| JC7 |
As JC5 but mtrR::kan
|
This study |
| JC8 |
As JC7 but farR+ delivered using vector pGCC3 |
This study |
| JC9 |
As JC7 but mtrR+ delivered using vector pGCC4 |
This study |
| M7AZ |
As M7 but with a nadA promoter translationally-fused to lacZ and delivered by pLES94 termed nadA-lacZ
|
This study |
| JC2AZ |
As JC2 but with a nadA-lacZ
|
This study |
| JC3AZ |
As JC3 but with a nadA-lacZ
|
This study |
| JC4AZ |
As JC4 but with a nadA-lacZ
|
This study |
| JC5AZ |
As JC5 but with a nadA-lacZ
|
This study |
| JC6AZ |
As JC6 but with a nadA-lacZ
|
This study |
| JC7AZ |
As JC7 but with a nadA-lacZ
|
This study |
| JC8AZ |
As JC8 but with a nadA-lacZ
|
This study |
| JC9AZ |
As JC9 but with a nadA-lacZ
|
This study |
| M7RZ |
As M7 but with an mtrR promoter translationally-fused to lacZ and delivered by pLES94 termed mtrR-lacZ
|
This study |
| M7FZ |
As M7 but with an farR promoter translationally-fused to lacZ and delivered by pLES94 termed farR-lacZ
|
This study |
| JC2FZ |
As JC2 but with a farR-lacZ
|
This study |
| JC3FZ |
As JC3 but with a farR-lacZ
|
This study |
| JC4FZ |
As JC4 but with a farR-lacZ
|
This study |
|
Escherichia coli
|
|
| DH5α |
[F− Φ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17(rΚ
− mΚ
+) deoR thi-1 supE44 λ−
gyrA96 relA1] |
[41]
|
| Top10 |
[F−
mcrA Δ(mrr-hsdRMS-mcrBC)Φ80 lacZΔM15 ΔlacX74 recA1 deoR araD139 Δ(ara-leu)7697 galU galK rpsL (Strr
endAI nupG)] |
Invitrogen |
|
Plasmids
|
|
|
| pCR®2.1-TOPO® |
pUC-derived cloning vector; AmpR
|
Invitrogen |
| pUC18K |
Source of aphA-3 cassette; KanR
|
[36]
|
| pHP45Ω |
Source of spc cassette; SpcR
|
[42]
|
| pLES94 |
pUC18K-derivative allowing a translational fusion to a promoterless lacZ fusion and insertion between neisserial proA and proB genes. |
[23]
|
| pGCC3 |
NCIS vector for insertion of genes directed under their native promoter between neisserial lctp and aspC genes. |
[37]
|
| pGCC4 |
As pGCC3 except inserted genes are directed under an IPTG-inducible promoter |
[37]
|
| pMal-c2 |
IPTG-inducible expression vector for fusion of proteins to maltose binding protein (MBP) and cytoplasmic expression |
NE Biolabs |
