Table 1. Analysis of cell surface markers on macrophage subsets.

	Without LPS			With LPS
Markers	IL-34-Mφ	M-CSF-Mφ	GM-CSF-Mφ	IL-34-Mφ	M-CSF-Mφ	GM-CSF-Mφ
CD14	++++	++++	+++	+++	+++	++++
CD163	+++++	+++++	++	+++++	+++++	+++
CD80	−	−	+	−	−	+
CD86	+	+	+	+	+	++
B7H1	−	−	−	+	+	+
ILT3	++++	++++	+++	++	++	+
CD54	+	+	++	++	+++	+++
MHC 1	+++	+++	+++	+++	+++	++++
MHC 2	++++	++++	+++	+++	+++	++++
CD206	−	−	+++	+	+	++

The expression of the indicated markers was analyzed by FACS on IL-34-Mφ, M-CSF-Mφ and GM-CSF-Mφ, stimulated or not for 48 h with 200 ng/ml LPS. Results are expressed as mean fluorescence intensity values (after subtraction of the MFI obtained with the isotype control mAb) and are representative of 4 separate experiments. +++++ indicates MFI greater than 1000; ++++, MFI greater than 400 and less than 1000; +++, MFI greater than 200 and less than 400; ++, MFI greater than 100 and less than 200; +, MFI greater than 20 and less than 100 and, − indicates MFI less than 20.