Figure 2. Species specificity of Kit activation by Kit ligand.

Plasmids expressing mouse KIT (mKIT), zebrafish Kita (A) or Kitb (B) were transfected into the COS-1 cells. After treatment with recombinant mouse KITL (mKITL) for 10 min, the phosphorylation of Kit was detected by an antibody recognizing the tyrosine phosphorylation site in the intracellular domain. Plasmid pCMV-Script (Vector) was used as a negative control. Bactin was used as the internal control for all samples. p-Kit, Kit phosphorylation; +, recombinant ligand proteins were added or cells were transfected with specific receptor plasmids; −, no recombinant ligands or transfection with receptor plasmids.