Figure 5. Dose response activation of Kita and Kitb by Kit ligands.

The COS-1 cells expressing Kita and Kitb were treated with conditioned media containing recombinant Kitlga (A) and Kitlgb (B) for 10 min followed by Western blot analysis for Kit phosphorylation (left) and characterization of Kitlga and Kitlgb potency (right). The ED50 for each ligand was defined as 1 Unit. p-Kit, Kit phosphorylation.