Skip to main content
. 2013 Feb 8;8(2):e56192. doi: 10.1371/journal.pone.0056192

Figure 9. Effects of EGF and IGF-I on MAPK phosphorylation in immature intact follicles or ovulated mature oocytes.

Figure 9

The immature intact full-grown follicles (A) or ovulated mature oocytes (B) were treated with human EGF or IGF-I for 10 min followed by Western blot analysis for MAPK (Erk1/2) phosphorylation. The Western blot image is shown on the top of each graph and the densitometric quantification is at the bottom. The data were normalized to the total Erk1 or Erk2 and expressed as the fold change compared to the control (CTL) (mean ± SEM, n = 3). **/++ P<0.01; ***/+++ P<0.001. p-Erk1/2, phosphorylated Erk1/2; t-Erk1/2, total Erk1/2.