Figure 13. Effects of recombinant zebrafish Kitlga and Kitlgb on oocyte maturation.

Full-grown immature follicles were pretreated for 6 h with Kitlga (200 µl Kitlga conditioned medium+200 µl control medium per ml), Kitlgb (400 µl/ml Kitlgb conditioned medium) and control medium (CTL, 400 µl per ml)), respectively, followed by treatment with DHP (5 ng/ml) for 9 h. Germinal vesicle breakdown (GVBD) was scored at different time points and expressed as the percentage (mean ± SEM, n = 4).