Table 1. Mitochondrial and nuclear DNA sequences from Bursaphelenchus xylophilus (Bx) and B. mucronatus (Bm) isolates used in the present study.
Isolate code | Isolate origin | Culture | GenBank accession numbers (genetic marker) |
BxCa188 | Canada (Quebec) | AY508071 (COI), AY508108 (28S rRNA) | |
BxCa185 | Canada | AY508068 (COI), AY508105 (28S rRNA) | |
BxCa187 | Canada (New Brunswick) | AY508070 (COI), AY508107 (28S rRNA) | |
BxCaBC | Canada (British Columbia) | AB108439 (ITS-2) | |
BxCaFIDS | Canada | AB108441 (ITS-2) | |
BxCaQ52A | Canada (Quebec) | AB108444 (ITS-2) | |
BxCaStJ | Canada | AB108445 (ITS-2) | |
BxCaBXCANADA | Canada | EF446946 (ITS-2), EF446935 (28S rRNA) | |
BxCaCA | Canada | JF317229 (ITS-2), JF317239 (28S rRNA) | |
BxCaQ52 | Canada | JF317230 (ITS-2), JF317241 (28S rRNA) | |
BxCaBC2 | Canada | JF317231 (ITS-2), JF317240 (28S rRNA) | |
BxCaBxCAN | Canada | EU295503 (28S rRNA) | |
BxUSA618 | USA | 2008 | JN596443 (COI), JN596460 (ND5), JN596470 (s-rRNA) |
BxUSA745 | USA | 2008 | JN596444 (COI), JN596461 (ND5) |
BxUSA30697 | USA | JF317255 (COI) | |
BxUSA4049 | USA | JF317256 (COI), JF317232 (ITS-2) | |
BxUSA121AD | USA | JF317257 (COI), JF317234 (ITS-2), JF317244 (28S rRNA) | |
BxUSAMO | USA | AB108442 (ITS-2) | |
BxUSABXUSA2 | USA | EF446951 (ITS-2), EF446940 (28S rRNA) | |
BxUSAUS10 | USA | JF317243 (28S rRNA) | |
BxUSA39906 | USA | JF317245 (28S rRNA) | |
BxJT4 | Japan (Iwate) | <2008 | JN596430 (COI), JN596458 (ND5), AB108446/AB277207 (ITS-2) |
BxJ10 | Japan | <2001 | JN596429 (COI), JN596459 (ND5), JN596466 (s-rRNA) |
BxJpS10 | Japan (Shimane) | AB067766 (COI), AB277206/U92464 (ITS-2) | |
BxJp186 | Japan (Mito) | AY508069 (COI), AY508106 (28S rRNA) | |
BxJpAB050051 | Japan (Akita, Ohmori) | AB050051 (ITS-2) | |
BxJpAB050052 | Japan (Niigata, Murakami) | AB050052 (ITS-2) | |
BxJpAB050053 | Japan (Ibaraki, Tsukuba) | AB050053 (ITS-2) | |
BxJpKyoto1 | Japan (Kyoto) | AB050054 (ITS-2) | |
BxJpKyoto2 | Japan (Kyoto) | AB050055 (ITS-2) | |
BxJpAB050056 | Japan (Yamaguchi, Tokuyama) | AB050056 (ITS-2) | |
BxJpAB050057 | Japan (Ehime, Imabari) | AB050057 (ITS-2) | |
BxJpAB050058 | Japan (Nagasaki, Shimabara) | AB050058 (ITS-2) | |
BxJpAB050059 | Japan (Okinawa, Kunigami) | AB050059 (ITS-2) | |
BxJpOk-2 | Japan (Okinawa) | AB108443 (ITS-2) | |
BxJpC14-5 | Japan (Chiba, Ichinomiya) | AB277203 (ITS-2) | |
BxJpOKD1 | Japan (Okayama, Okayama) | AB277205 (ITS-2) | |
BxJpBCMUBX18 | Japan (Aichi, Nissin, Iwasaki-cho) | AB294736 (ITS-2) | |
BxJpAY347913 | Japan | AY347913 (ITS-2) | |
BxJpBXJ1 | Japan | EF446943 (ITS-2), EF446934 (28S rRNA) | |
BxJpXylT4 | Japan | DQ356002 (28S rRNA) | |
BxJpBxJAP | Japan | EU295504 (28S rRNA) | |
BxCSD | China | <2009 | JN596428 (COI), JN596456 (ND5), JN596465 (s-rRNA) |
BxChBXC | China | AB108440 (ITS-2) | |
BxChAY347911 | China (Xiangshan, Zhejiang) | AY347911 (ITS-2) | |
BxChAY347912 | China (Nanjing, Jiangsu) | AY347912 (ITS-2) | |
BxChBXCNJ3 | China (Nanjing, Jiangsu) | EF446944 (ITS-2), EF446929 (28S rRNA) | |
BxChBXCAJ | China (Mingguang, Anhui) | EF446945 (ITS-2), EF446942 (28S rRNA) | |
BxChBXCSC | China (Changdao, Shandong) | EF446947 (ITS-2), EF446932 (28S rRNA) | |
BxChBXCNJ2 | China (Nanjing, Jiangsu) | EF446948 (ITS-2), EF446941 (28S rRNA) | |
BxChBXCGD | China (Dongguan, Guangdong) | EF446950 (ITS-2), EF446933 (28S rRNA) | |
BxChBXCZZ | China (Zhoushan, Zhejiang) | EF446952 (ITS-2), EF446937 (28S rRNA) | |
BxChXM_1 | China (Fujian) | EU259322 (ITS-2) | |
BxChDQ364687 | China (Xiangshan) | DQ364687 (28S rRNA) | |
BxChBXCNJ1 | China (Nanjing, Jiangsu) | EF446930 (28S rRNA) | |
BxChBXCNJ4 | China (Nanjing, Jiangsu) | EF446931 (28S rRNA) | |
BxChBxLYG | China (Lianyungang) | EU295491 (28S rRNA) | |
BxTaNe6/05 | China (Taiwan) | AM179515 (ITS-2) | |
BxTaTWRC | China (Taiwan) | JF317242 (28S rRNA) | |
BxKAS | South Korea | <2008 | JN596431 (COI), JN596457 (ND5), JN596467 (s-rRNA) |
BxPt11AS | Portugal (Alcácer do Sal) | 2005 | JN596432 (COI), JN596447 (ND5) |
BxPt15SC | Portugal (Santiago do Cacém) | 2007 | JN596433 (COI), JN596448 (ND5) |
BxPt17AS | Portugal (Alcácer do Sal) | 2007 | JN596434 (COI), JN596449 (ND5), JN596468 (s-rRNA) |
BxPt19SCD | Portugal (Santa Comba Dão) | 2008 | JN596435 (COI), JN596450 (ND5), JN596469 (s-rRNA) |
BxPt21T | Portugal (Tábua) | 2008 | JN596436 (COI), JN596451 (ND5) |
BxPt56M | Portugal (Mealhada) | 2009 | JN596438 (COI), JN596446 (ND5) |
BxPt60OH | Portugal (Oliveira do Hospital) | 2009 | JN596437 (COI), JN596445 (ND5) |
BxPtHF | Portugal (Herdade de Ferraria) | AB277204 (ITS-2) | |
BxPtTroia | Portugal (Troia) | AB277208 (ITS-2) | |
BxPtPT1w | Portugal (Pegões) | AM157747 (ITS-2), AM396580 (28S rRNA) | |
BxPtBXPOT | Portugal | EF446949 (ITS-2), EF446936 (28S rRNA) | |
BxMad1F | Portugal (Madeira Island) | 2010 | JN596439 (COI), JN596452 (ND5) |
BxMad2M | Portugal (Madeira Island) | 2010 | JN596440 (COI), JN596453 (ND5) |
BxMad3F | Portugal (Madeira Island) | 2010 | JN596441 (COI), JN596454 (ND5) |
BxMad4SV | Portugal (Madeira Island) | 2010 | JN596442 (COI), JN596455 (ND5) |
BxSpEFA1 | Spain | HQ646254 (ITS-2) | |
BxMe39906-1 | Mexico | JF317253 (COI) | |
BxMe39906-2 | Mexico | JF317254 (COI) | |
BxMe39906 | Mexico | JF317233 (ITS-2) | |
BmJpM | Japan | AB067765 (COI) | |
BmJp163 | Japan | AY508049 (COI), AY508086 (28S rRNA) | |
BmJp424B | Japan | JF317260 (COI), JF317235 (ITS-2), JF317246 (28S rRNA) | |
BmChAY347915 | China (Hong Kong) | AY347915 (ITS-2) | |
BmChAY347916 | China (Fuyang, Zhejiang) | AY347916 (ITS-2) | |
BmChBMCSC | China (Zhoushan, Zhejiang) | EF446953 (ITS-2), EF446938 (28S rRNA) | |
BmChXM | China (Fujian) | EU296624 (ITS-2) | |
BmKo39571 | South Korea | JF317261 (COI), JF317236 (ITS-2), JF317247 (28S rRNA) | |
BmKoAY347914 | South Korea | AY347914 (ITS-2) | |
BmPt1 | Portugal | 2008 | JN596463 (s-rRNA) |
BmPt2 | Portugal | 2008 | JN596464 (s-rRNA) |
BmSp860A | Spain | JF317262 (COI) | |
BmG1 | Germany | <2001 | JN596427 (COI), JN596462 (s-rRNA) |
BmG166 | Germany (Zusmarshausen) | AY508052 (COI), AY508089 (28S rRNA) | |
BmG167 | Germany (Grunberg) | AY508053 (COI), AY508090 (28S rRNA) | |
BmG168 | Germany (Zusmarshausen) | AY508054 (COI), AY508091 (28S rRNA) | |
BmFi165 | Finland | AY508051 (COI), AY508088 (28S rRNA) | |
BmNo164 | Norway (Hanestad) | AY508050 (COI), AY508087 (28S rRNA) | |
BmUk38624 | Ukraine | JF317258 (COI) | |
BmUk53106 | Ukraine | JF317238 (ITS-2) | |
BmRuBMRUSSIAN | Russia | EF446939 (28S rRNA) | |
BmIs5459 | Israel | JF317237 (ITS-2) | |
BmBr4228 | Brazil | JF317259 (COI) |
The list includes sequences from the mitochondrial cytochrome c oxidase subunit I (COI), NADH dehydrogenase subunit 5 (ND5) and small subunit ribosomal RNA (s-rRNA) genes and the nuclear internal transcribed spacer 2 (ITS-2) and 28S ribosomal RNA gene (28S rRNA). The accession numbers in bold indicate new sequences obtained in this work.