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. 2013 Feb 8;8(2):e56273. doi: 10.1371/journal.pone.0056273

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© 2013 Shroff et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Ang-1 was detected in the media of vessels from both pre-dialysis CKD (A) and dialysis patients (B); no differences in staining intensity were observed between the two groups (C). Ang-2 was immunodetected in both the media and endothelia (arrows) in pre-dialysis CKD (D) and dialysis (E) vessels with similar intensity (F). The endothelial later was also positive for von Willebrand factor (arrows, G and H). VEGF-A immunostaining was prominent in the media of pre-dialysis CKD vessels (I), but was significantly decreased in dialysis patients (J and K). All fields taken with ×40 objective.