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. 2013 Feb 8;8(2):e56273. doi: 10.1371/journal.pone.0056273

Figure 6. Effect of uric acid on Ang-2 secretion in HUVECs.

Figure 6

A) Both non-stimulated and uric acid stimulated HUVECs expressed the mRNA for the transporter Urat1 but not Oat1-4; they were also positive for Ang-1, Ang-2, Tie-1, Tie-2 and Tlr4. Sizes were determined using a 100 bp marker (m), positive (+ive) controls consisted of total kidney cDNA and negative controls were without cDNA addition. (B) Uric acid stimulation for 15 minutes, but not 72 hours (C) led to elevated Ang-2 secretion in the conditioned media of HUVEC cells. Within the cells, uric acid stimulation led to a decreased abundance of Ang-2 mRNA after 6 hours of stimulation (D). a = p<0.05 compared with controls, b = p<0.01 compared with controls, c = p<0.001 compared with controls, d = p<0.01 compared with HUVEC stimulated with 3 mg/dl uric acid, e = p<0.01 compared with HUVEC stimulated with 6 mg/dl uric acid, f = p<0.05 compared with HUVEC stimulated with 9 mg/dl uric acid.