Figure 1.

Schematic view of the multi-stage multi-orifice flow fractionation (MS-MOFF) system. At the first MOFF stage, the RBCs and WBCs split into two positions, and the MCF-7 cells focused at the middle of the channel (inside). At the second MOFF stage, RBCs and WBCs directed to the side channel (outside), similar to the first stage. On the other hand, a few MCF-7 cells that travelled through the side channel at the first stage (non-selected target) were directed to the middle of the channel (inside).