Table 2. Sequences of ASO primers and corresponding annealing temperatures (bold nucleotides in the primers denote nucleotide changes corresponding to mutations).

*Primer name	Primer polarity	**Nucleotide change	5′–3′ sequence	$L(bp)	#A Tm(°C)
1. M244V-F	F	A1094G	GAACGCACGGACATCACCG	19	65.7
2. L248V	F	C1106G	ACCATGAAGCACAAGG	16	55
3. G250E	F	G1113A	GAAGCACAAGCTGGGCGA	18	56
4. Q252H(a)	F	G1120C	AGCTGGGCGGGGGCCAC	17	62
5. Q252H(b)	F	G1120T	AGCTGGGCGGGGGCCAT	17	62
6. Y253H	F	T1121C	GCTGGGCGGGGGCCAGC	17	62
7. Y253F	F	A1122T	CTGGGCGGGGGCCAGTT	17	55
8. E255K	F	G1127A	GCGGGGGCCAGTACGGGA	18	68
9. E255V	F	A1128T	GCGGGGGCCAGTACGGGGT	19	58
244 R	R(1–9)	–	GCCAATGAAGCCCTCGGAC	19
10. F311L	F	T932C	CACCCGGGAGCCCCCGC	17	62
–	R(10)	–	CCCCTACCTGTGGATGAAGT	20
11. T315I	F	C1308T	GCCCCCGTTCTATATCATCAT	21	63.4
12. F317L	F	C1315G	CCGTTCTATATCATCACTGAGTTG	24	54
315 R	R(11–12)		GGATGAAGTTTTTCTTCTCCAG	22
13. M343T	F	T1392C	GTGGTGCTGCTGTACAC	17	62
14. M351T	F	T1416C	CCACTCAGATCTCGTCAGCCAC	22	70
351 R1	R (13–14)		GCCCTGAGACCTCCTAGGCT	20
15. E355G	–	A1428G	GTCAGCCATGGAGTACCTAGG	21	56
16. F359V	F	T1439G	GAGTACCTAGAGAAGAAAAACG	22	50
351 R2	R (15–16)		ATGCCCAAAGCTGGCTTTG	19
17. H396R	F	A1551G	GGACACCTACACAGCCCG	18	62.5
369 R	R(17)		GGACACCTACACAGCCCG	18
18. F486S	F	T1821C	TCTGACCGGCCCTCCTC	17	62
486 R	R(18)		AGCTTTCTGGTCTCAGGA	18

^*Substitutions of amino acids; positions according to GenBank no. AAB60394for ABL type 1a.

^**Changes of nucleotide; positions according to GenBank no.M14752.

^$L (bp) = Primer length in base pairs.

^#A Tm =  Annealing temperature in degree Celsius.