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. 2013 Feb 8;8(2):e55827. doi: 10.1371/journal.pone.0055827

Figure 1. Generation of ApoA1 transgenic mice.

Figure 1

Overexpression of hApoA1 was induced using doxycycline-containing drinking water (Doxy+). Control mice were offered normal water (Doxy−). (A) RT-PCR analysis revealed hApoA1 mRNA only in the lungs of mice treated with doxycycline. (B) ApoA1 protein expression was significantly greater in the lungs of doxycycline-treated transgenic mice compared with those of wild-type or transgenic mice not treated with doxycycline (n = 6 mice/group). **p<0.01 compared with WT or Doxy(−) mice. (C) Detection of human ApoA1 in BAL fluids. ELISA was performed on the first 1-mL fraction of BAL fluid, with a detection limit of 3.13 ng/mL (dashed line). Values below the detection limit were treated as 0 (n = 6 mice/group). **p<0.01 compared with Doxy(−) mice. (D) Immunofluorescence analysis showed that ApoA1 was strongly expressed in the alveolar epithelium (white arrow heads) in the doxycycline-treated transgenic mice. Scale bar = 10 µm. H&E, hematoxylin and eosin.