Figure 2. Endo-metabolome analysis of S. cerevisiae BY4741 parental strain under ethanol stress.

Yeast cells were harvested during the exponential phase of growth (OD_{600 nm} = 1.0) in the same basal medium and supplemented with different ethanol concentrations (% v/v). (A) 2D score plot displaying the space formed by the two first principal components (R²X for PC1 equal to 0.792 and R²X for PC2 equal to 0.129), (B) 2D loading plot displaying the space formed by the two first principal components and presenting the characteristic bins for a set of metabolites and (C) Relative abundance of different metabolites in the endo-metabolome of S. cerevisiae BY4741 cells harvested during the exponential phase of growth (OD_{600 nm} = 1.0) in the presence of different ethanol concentrations (0, 2, 4 and 6% v/v). Each plot displays the variation in the relative abundance of a representative bin in the spectrum for each amino acid. Experimental values are means of six independent experiments with standard deviation error bars. Key: 1 Glycerol, 2 L-arginine, 3 NAD⁺, 4 NADP⁺, 5 L-tyrosine, 6 L-histidine, 7 Trehalose, 8 NADH, 9 Fumaric acid, 10 L-proline, 11 L-phenylalanine, 12 L-asparagine, 13 Citric acid, 14 L-serine, 15 Succininic acid, 16 L-aspartic acid, 17 Malic acid, 18 L-glutamine, 19 L-alanine, 20 L-lysine, 21 L-glycine, 22 L-threonine, 23 L-valine, 24 L-methionine, 25 L-leucine, 26 L-glutamic acid and 27 L-ornithine.