Figure 4. Comparison between the endo-metabolome of BY4741 parental strain and of fps1Δ deletion mutant.

Yeast cells were harvested during the exponential phase of growth (OD_{600 nm} = 1.0) in the same basal medium supplemented with 0 or 2% v/v of ethanol. (A) 2D score plot displaying the space formed by the two first principal components (R²X for PC1 equal to 0.525 and R²X for PC2 equal to 0.321), (B) 2D loading plot displaying the space formed by the two first principal components (with characteristic bins for a set of metabolites) and (C) relative abundances of different metabolites in the endo-metabolome of the parental strain and the fps1Δ deletion strain during the exponential growth phase (OD_{600 nm} = 1.0) in the presence of different ethanol concentrations (0 and 2% v/v). Experimental values are means of six independent experiments with standard deviation error bars. Key: 1 NAD⁺, 2 NADP⁺, 3 L-tyrosine, 4 Fumaric acid, 5 L-phenylalanine, 6 Citric acid, 7 Succininic acid, 8 Glycerol, 9 L-glutamic acid, 10 L-leucine, 11 L-methionine, 12 L-alanine, 13 L-ornithine, 14 L-threonine, 15 L-valine,16 L-glutamine, 17 L-aspartic acid, 18 L-malic acid, 19 L-histidine, 20 L-serine, 21 L-asparagine, 22 L-lysine, 23 Thiamine, 24 NADH and 25 L-proline.