Table 2. Oligonucleotide primers used to amplify bacterial and archaeal 16S rRNA genes, ciliate 18S rRNA genes and anaerobic fungal ITS1 genes.

Name	Adaptor	(Linkera +) Primer sequence [5′ → 3′]	Region	Length [bp]	Annealing [°C]	Abbreviation	Reference
ArBa515F	B	(TA) GTG CCA GCM GCC GCG GTA A	16S	∼290	52	ArBa	[26]
ArBa806R	Ab	(AC) GGA CTA CVS GGG TAT CTA AT					[57]
Ba9F	B	GAG TTT GAT CMT GGC TCA G	16S	∼525 (+73)	52	BaL	[58]
Ba515Rmod1	Ab	CCG CGG CKG CTG GCA C					modified from [59]
Ba27F	B	AGA GTT TGA TCC TGG CTC AG	16S	∼365	52	BaS	[60]
Ba338R	Ac	TGC TGC CTC CCG TAG GAG T					modified from [61]
Ar915aF	Ab	AGG AAT TGG CGG GGG AGC AC	16S	∼492 (+73)	59	ArL	[62]
Ar1386R	B	GCG GTG TGT GCA AGG AGC					[63]
Ar344F	Ab	ACG GGG YGC AGC AGG CGC GA	16S	∼148 (+73)	60	ArS	[64]
Ar519R	B	TTA CCG CGG CKG CTG					[65]
RP841F	B	(AA) GAC TAG GGA TTG GAG TGG	18S	∼511 (+73)	54	RP	[21]
Reg1302R	Ab	(TC) AAT TGC AAA GAT CTA TCC C					[66]
MN100F	Ab	TCC TAC CCT TTG TGA ATT TG	ITS1	∼250 (+73)	50	RF	[67]
MNGM2	B	CTG CGT TCT TCA TCG TTG CG					[67]

^aLinker sequences were designed according to Walters et al. [28].

^bGolay barcodes of 12-nucleotides in length were used in combination with this primer.

^cHamming barcodes of 8-nucleotides in length were used in combination with this primer.