Figure 3. Sequence conservation across intron 16 of multi species ACE genes.

(a) Plot of conservation spanning exons 15 to 18 between the developed equine ACE sequence and the reference sequences from the dog, human, elephant, rabbit and mouse. Pink coloured regions are >70% conserved between the horse and query sequence; while the dark blue regions are annotated exons. Pink conserved peaks are clearly visible in intron 16. The positions of common equine SNPs are marked with black arrows, and the human I/D variant is noted in blue. The human intron sequence analysed here was of the deletion allele. (b) Aligned intron sequence from six species showing the conserved nucleotides highlighted in yellow. The position of SNP rs4338 that occurs in the middle of the human CNE is highlighted in green. (c) Results of the rVISTA scan showing likely motif binding sites (green bars) for the three identified transcription factors.