Table 1. Genotyping conditions for nine common equine ACE gene polymorphisms.

SNP	Position	Variant	Breeds	Genotyping Method
1	Intron 5	c.868+25A>G	AR, TB	Eliminates NspI restriction site
2	Intron 8	c.1363+146G>T	AR, TB	Introduces BamHI restriction site
3, 4	Intron 16	c.2326+89C>G; c.2326+178G>A	AR, TB; AR, TB, SB, HH	Sequencing
5	Intron 16	c.2326–583G>T	AR, TB, SB, HH	Sequencing
6, 7	Intron 20	c.2933+58G>A>C; c.2933+115G>T	AR, TB; AR, TB	Sequencing
8	Intron 21	c.3157+39C>A	TB, AR, HH, BAC	Sequencing
9	Exon 26	p.Arg1290His	HH	Eliminates AciI restriction site

The breeds column indicates the breeds the polymorphism was indentified in this study. Variant annotation was according to the guidelines of the Human Genome Variation Society. Primer sequences are listed in Table S1.