Table 1. Genotyping conditions for nine common equine ACE gene polymorphisms.
SNP | Position | Variant | Breeds | Genotyping Method |
1 | Intron 5 | c.868+25A>G | AR, TB | Eliminates NspI restriction site |
2 | Intron 8 | c.1363+146G>T | AR, TB | Introduces BamHI restriction site |
3, 4 | Intron 16 | c.2326+89C>G; c.2326+178G>A | AR, TB; AR, TB, SB, HH | Sequencing |
5 | Intron 16 | c.2326–583G>T | AR, TB, SB, HH | Sequencing |
6, 7 | Intron 20 | c.2933+58G>A>C; c.2933+115G>T | AR, TB; AR, TB | Sequencing |
8 | Intron 21 | c.3157+39C>A | TB, AR, HH, BAC | Sequencing |
9 | Exon 26 | p.Arg1290His | HH | Eliminates AciI restriction site |
The breeds column indicates the breeds the polymorphism was indentified in this study. Variant annotation was according to the guidelines of the Human Genome Variation Society. Primer sequences are listed in Table S1.