FIG. 1.

PCR amplification of 400-bp region of IS900 from DNA obtained from bovine milk spiked with 1 to 10⁴ M. avium subsp. paratuberculosis organisms. DNA was extracted from bovine milk spiked with M. avium subsp. paratuberculosis organisms after IMS of organisms and bead beating. Lane 1, 100-bp marker; lane 3, positive PCR control; lane 4, DNA processing control; lanes 5 and 11, negative milk controls; lanes 6 to 10, DNA extracted from milk samples containing 1 to 10⁴ organisms; lanes 12 to 16, DNA extracted from milk samples containing 1 to 10⁴ organisms.