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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: J Immunother. 2013 Feb;36(2):112–123. doi: 10.1097/CJI.0b013e3182811ce9

Figure 3. In-process testing of genetically modified T cells harvested at Day 28 of culture on aAPC.

Figure 3

(a) The assay to screen for lack of autonomous cell growth showed loss of T cells in the absence of antigen (aAPC clone #4) and IL-2. (b) Integrated SB11 transposase sequence was not detected in T-cell genome. Jurkat cell stably expressing SB11 transposase served as a positive control. (c) Messenger RNA coding for SB11 was not detected in T cells. Total cellular RNA from propagated CAR+ T cells was subjected to cDNA synthesis followed by PCR for the presence of SB11 transposase. Jurkat cell stably expressing SB11 transposase served as a positive control.