Figure 1.

Systems approach to enhance microbial ROS production. (left) Methodology for the development and validation of an ensemble of systems-level models of E. coli metabolism for estimation of basal ROS production. ROS-generating reactions were incorporated into a metabolic reconstruction and FBA framework¹⁷. Network perturbations via single-gene knockouts were performed in silico using FBA to identify alterations that affect ROS production. (right) In silico predictions were evaluated experimentally by generating mutants and measuring their ROS production and susceptibility to killing by oxidants and antibiotics.