Figure 2.

In silico predictions and experimental measures of H₂O₂ and O₂⁻ levels. (a) Predicted H₂O₂ levels of various mutants compared to wildtype. Blue designates strains whose mean H₂O₂ production levels were simulated to be >5% higher than wildtype over both ensembles, whereas yellow designates strains whose mean H₂O₂ production levels were simulated to be <5% higher than wildtype over both ensembles. (b) Predicted O₂⁻ levels of various mutants compared to wildtype. Blue designates strains whose mean O₂⁻ production levels were simulated to be >5% higher than wildtype over both ensembles, whereas yellow designates strains whose mean O₂⁻ production levels were simulated to be <5% higher than wildtype over both ensembles. (c) Experimentally measured relative fluorescence/OD600 of strains with the H₂O₂-sensitive reporter (dps promoter-gfp). Blue designates strains that were experimentally measured to have increased levels of H₂O₂ compared with wildtype (P-value < 0.05), whereas yellow designates strains that were experimentally measured to have levels of H₂O₂ that do not exceed those of wildtype. (d) Experimentally measured relative fluorescence/OD600 of strains with the O₂⁻-sensitive reporter (soxS promoter-gfp). Blue designates strains that were experimentally measured to have increased levels of O₂⁻ compared with wildtype (P-value < 0.05), whereas yellow designates strains that were experimentally measured to have levels of O₂⁻ that do not exceed those of wildtype. * denotes genes that are essential in our media conditions. Grey denotes genes that were not experimentally examined (for consistency between diagrams, these genes were also denoted by grey in a and b, although in silico predictions were computed).