FIG. 2.

Identification of bacteria from pure cultures by on-chip PCR. (Top panel) Genomic DNA was extracted from pure cultures and subjected to on-chip PCR with the DNA chip depicted in Fig. 1. The fluorescent image obtained in experiments with R. equi is shown. (Bottom panel) Quantitative fluorescence (Cy5) profile of the DNA chip analyzed. The average fluorescence intensities from all replicate spots (y axis) are plotted for all corresponding solid-phase primers. The names of all primers with positive fluorescence signals above the threshold (10,000 arbitrary units) are shown.