TABLE 1.

Bacterial strains used in this study and their corresponding specific on-chip PCR solid-phase primers

Species	Strain no. and sourcea	Specific solid-phase primers	Solid-phase primer patternc
Actinobacillus equuli	CIP 103284T	4a	4a
Bordetella bronchiseptica	DSM 13414T	4b	4b
Citrobacter diversus	DSM 4570T	6b, 6c	5b, 5c, 6b, 6c, 7a
Citrobacter freundii	DSM 30039T	6c	5b, 5c, 6c, 7a, 7c
Citrobacter koseri	DSM 4595T	6c	5b, 5c, 6a, 6b, 6c, 7a
Enterobacter cloacae	DSM 30054T	9c	5b, 5c, 6a, 6c, 7c, 9c
Enterobacter sakazakii	DSM 4485T	9c	5c, 9c
Escherichia coli	DSM 30083T	5a, 5b, 5c, 6a	5a, 5b, 5c, 6a, 6c, 7a, 7c
Klebsiella oxytoca	DSM 5175T	8b	5b, 6a, 8b
Klebsiella pneumoniae	DSM 30104T	8a	5a, 5c, 9c, 8a
Pantoea agglomerans	DSM 3493T	9b	9b
Proteus mirabilis	DSM 4479T	8c, 9a	5c, 8c, 9a
Proteus vulgaris	DSM 13387T	8c, 9a	5c, 8c, 9a, 7c
Pseudomonas aeruginosa	DSM 50071T	3c	3c
Pseudomonas fluorescens	DSM 50090T	—b	No signal
Rhodococcus equi	DSM 20307T	3a, 3b	3a, 3b
Salmonella enterica serovar Abortusequi	CIP 55.131	7b, 7c	5c, 6c, 7a, 7b, 7c
Staphylococcus aureus	DSM 20231T	2a, 2b, 2c	2a, 2b, 2c
Staphylococcus intermedius	DSM 20373T	2c	2c
Streptococcus equi subsp. equi	DSM 20561T	1a, 1c	1a, 1b, 1c
Streptococcus equi subsp. zooepidemicus	DSM 20727T	1a, 1b	1a, 1b
Taylorella equigenitalis	DSM 10668T	4c	4c

^aCIP, Collection de l'Institut Pasteur, Institute Pasteur; DSM, German Collection of Microorganisms and Cell Cultures.

^b—, no specific solid-phase primer targeted at this species was present on the DNA chip.

^cThe reacting primer patterns observed after on-chip PCR with extracted DNA from pure cultures.