TABLE 3.
Comparison of results obtained by on-chip PCR identification and conventional bacteriology for 21 clinical samples
| Result by on-chip PCR | No. (%) of samples by on-chip PCR | Result by conventional bacteriology | No. of samples by conventional bacteriology; |
|---|---|---|---|
| Congruent results | 47 (82.5) | ||
| Identical identification | 21 | ||
| Staphylococcus spp. | 7 | Staphylococcus spp. | 3 |
| Coagulase-positive staphylococci | 4 | ||
| Staphylococcus aureus | 2 | Coagulase-positive staphylococci | 2 |
| Streptococcus equi | 5 | Streptococcus equi | 3 |
| Beta-hemolytic Streptococcus sp. | 1 | ||
| Streptococcus spp. | 1 | ||
| Escherichia coli | 6 | Escherichia coli | 6 |
| Bacillus spp. | 1 | Aerobic spore-forming bacteria | 1 |
| Not represented (true negative) | 26 | Alpha-hemolytic Streptococcus sp. | 7 |
| Streptococcus spp. | 1 | ||
| Aerobic spore-forming bacteria | 5 | ||
| Neisseria spp. | 2 | ||
| Acinetobacter spp. | 5 | ||
| Coryneform bacteria | 6 | ||
| Conflicting results | 10 (17.5) | ||
| On-chip PCR failure (false negative) | 4 | Staphylococcus aureus | 1 |
| Coagulase-positive staphylococci | 2 | ||
| Streptococcus equi | 1 | ||
| No identical identification | 6 | ||
| No fluorescence signal | 1 | Pantoea agglomerans | 1 |
| Enterobacter spp. | 2 | No growth | 2 |
| Streptococcus equi | 2 | No growth | 2 |
| Pseudomonas aeruginosa | 1 | No growth | 1 |