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. 2013 Feb 5;110(6):E445–E446. doi: 10.1073/pnas.1220586110

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Fig. 2. — GC content differs according to caste- and developmentally biased gene expression in two ant species. A comparison of third codon position GC content (GC3) based on gene expression differences between bodies of virgin queens and workers from Camponotus floridanus and Harpegnathos saltator is made. According to Wilcoxon rank-sum tests, C. floridanus worker-biased genes differ significantly in GC3 from nonsignificantly biased genes (P < 0.0001) and queen-biased genes (P < 0.0001) (A), and H. saltator worker-biased genes differ significantly in GC3 from nonsignificantly biased genes (P < 0.0001) and queen-biased genes (P = 0.0006) (B). C. floridanus larval-biased genes differ significantly in GC3 from nonsignificantly biased genes (P < 0.0102) and from egg-biased genes (P < 0.0001) (C), and H. saltator larval-biased genes differ significantly in GC3 from nonsignificantly biased genes (P < 0.0001) and egg-biased genes (P < 0.0001) (D). To approximate pairwise differential expression calls from these unreplicated RNA-seq data, we used NOISeq (5) to assign significantly biased categories based on simulated replication.