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(A) Major substructures I–III were inferred from COSY, HSQC, HMBC, and ADEQUATE experiments. (B) Substructures were putatively linked via HMBC and ADEQUATE correlations. The xanthene framework was confirmed by 13C J-coupling measurements in the amino congeners of mutaxanthenes (D 4, E 5), generated by treatment with ammonium acetate, which were sharper than the A–C and afforded confirmation of the complete carbon framework of mutaxanthenes. (C) Proposed biosynthesis of mutaxanthenes.
