Fig. 3.

JNK1 regulates VEGF secretion in a murine model of CNV. (A) Choroidal tissues were collected at 3 d after laser administration, placed in 100 μL of RPMI medium supplemented with 10% FCS in 96 wells/ plate, and then kept at 37 °C for 16 h. VEGF levels were analyzed in the supernatants of homogenized tissue by ELISA. Results are averages of two experiments using at least four mice per genotype. Results are expressed as means ± SEM, *P < 0.05 vs. WT. (B–G) Choroidal tissue from either WT (B–D) or Jnk1^−/− (E–G) mice, subjected to laser-CNV and collected 3 d after laser treatment, were stained by immunofluorescence with isolectin (B and E) and anti-VEGF antibody (C and F, arrows). D and G show VEGF, isolectin, and DAPI overlapping staining. Original magnification, 200×.