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. 2013 Jan 22;110(6):2377–2382. doi: 10.1073/pnas.1221729110

Fig. 5.

Fig. 5.

JNK1 regulates apoptosis in a murine model of CNV. (A) WT mice (n = 10) were given an intravitreal injection of 1 μL of pan-caspase inhibitor Z-VAD-FMK (20 μM) right after laser treatment. An equal volume of PBS was given in the fellow eye as a control. Ten days after laser treatment, mice were killed and choroidal flat mounts generated. FITC-conjugated isolectin was used to immunolabel the CNV. Flat mounts were examined and CNV quantified. Results are expressed by CNV area (μm2) as means ± SEM, *P < 0.01 vs. PBS. (B) The retina of Jnk1−/− and WT control mice were examined for apoptosis by an in situ TUNEL assay with retinal cross-sections obtained 6, 12, and 24 h after laser-CNV treatment. Results are expressed as number of apoptotic cells within the 20× viewing area (n = 3, means ± SEM, *P < 0.01 vs. WT). (C–H) Representative images of retinal cross-sections of WT control (C–E) and Jnk1−/− (F–H) mice stained by an in situ TUNEL assay 6, 12, and 24 h after laser-CNV treatment (arrows indicate the positive TUNEL staining). Original magnification, 200×.