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Amino acid sequence of FGF-1, Symfoil-4P, PV1, and PV2 mutants (single letter code). The sequences are aligned by the threefold axis of rotational symmetry internal to the β-trefoil fold (i.e., each line is one trefoil-fold repeat element within the overall β-trefoil structure). The numbering of Symfoil-4P, PV1, and PV2 amino acids is based upon corresponding positions in FGF-1. The shaded positions identify amino acids belonging to the prebiotic set.
