Fig. 4.
PRKD2 mediates cell cycle effects of BCR-ABL in CML cells. (A) In vitro colony forming assay (in the absence of added cytokines) of WT or Gabpafl/fl mouse bone marrow infected with the indicated viruses. (B and C) Knockdown of PRKD2 by lentiviral infection of K562 cells with scrambled (NS) or two different shRNAs directed against PRKD2, analyzed by (B) immunoblotting with antibodies against PRKD2, phospho-PRKD at S744 and S748 (pp-PRKD), or β-Actin; and (C) cell cycle analysis by propidium iodide staining. (D–F) K562 cells grown with indicated duration and concentration of CRT0066101, analyzed by (D) immunoblotting with antibodies against PRKD1, PRKD2, and phospho-PRKD at S916 (p-PRKD), (E) growth by MTT assay, and (F) cell cycle analysis.