Fig. 3.

TcBuster transposition in mammalian cells. The transposition of TcBuster, hyperactive Sleeping Beauty, and piggyBac elements containing antibiotic resistance genes from transfected plasmids to the HeLa genome was measured by selection for antibiotic-resistant cells; the frequency of transposition of TcBuster transposition was compared with the frequency of a hyperactive Sleeping Beauty (HSB16) and piggyBac using different amounts of transposase expression plasmid and the transposon-containing donor plasmid.