Fig. 7.

TcBuster_CO transposition in yeast. (A) Integration assays that measure the transposition of a TcBuster transposon from a plasmid into the host genome by assaying plasmid-free cells for the presence of a transposon-encoded marker are shown. The transposase is expressed from a plasmid GALS promoter and thus, is induced in the presence of galactose. TcBuster_CO V596A has an amino acid change comparable with the hyperactive AeBuster1 V597A mutant. (B) Excision assays that measure the excision of a TcBuster transposon from a donor site in a plasmid URA3 gene by following reversion from Ura⁻ to Ura⁺ are shown. The transposase is expressed from a plasmid GALS promoter and thus, is induced in the presence of galactose. TcBuster_CO V596A has an amino acid change comparable with the hyperactive AeBuster1 V597A mutant.