Fig. 8.
hAT Buster element transposition in vitro. The products of in vitro transposition reactions using end-labeled oligonucleotides and circular plasmid targets are displayed on agarose gels. (A) Substrates, steps, and products of transposition. In SEJs, a single transposon end oligonucleotide joins to the target plasmid, giving a nicked circle; in DEJs, two transposon end oligonucleotides have joined to the target plasmid, giving a linear species. (B) Target joining and coupled cleavage using TcBusterCO transposase and end oligonucleotides as indicated are shown. (C) Target joining and coupled cleavage SPINON and end oligonucleotides as indicated are shown.