Fig. 4.

(A) DNA methylation at RMG1 promoter, a schematic representation of the RMG1 promoter (Upper) is presented where red arrows represent the position of primers used for bisulfite sequencing on WT and met1(^−/−) leaves (Left graph) or WT and ros1–4 leaves (Right). The regions analyzed with primers (1+2) and (3+4) contain 12 CG, six CHG, 56 CHH, and two CG, three CHG, and 27 CHH, respectively. Similar results were obtained in three independent experiments. (B) RMG1 mRNA levels in 5- to 6-wk-old WT or ros1–4 leaves treated with either water (−) or flg22 (+) for 3, 6, and 9 h with SD as in Fig. 1. Similar results were obtained in four independent experiments.