Figure 1.

Genome-wide binding profiles of Hmt1. A) Pie chart showing the fraction of Hmt1 binding sites that mapped to the indicated genomic feature. B) The percentage of each genomic feature bound by Hmt1 as compared to the total number of that feature in the nuclear genome. C) Directed ChIP validation of genes bound by Hmt1. ChIP signals were normalized to “No ORF”, which is an intergenic region on the right arm of Chromosome IV [68]. TRA1, CDC2, and SPS2 were negative controls. The use of ACT1 was to demonstrate the enrichment of Hmt1 occupancy within specific regions of a gene body. Lastly, a number of tDNAs binding sites were confirmed.