Fig. 6.

Uptake of ethidium bromide induced by ATP in three types of retinal monolayer cultures. Mixed and neuronal retinal cultures at E7C2 or glial cultures were treated with 3 mM ATP for 10–15 min in the presence of 5 μM ethidium bromide (EtBr) in Hanks’ balanced salt solution without Ca⁺⁺/Mg⁺⁺. After incubation, cells were washed and visualized under fluorescence illumination. a Representative photomicrographs of the three types of cultures labeled for β-tubulin III and the glial marker 2 M6. b Representative micrographs showing EtBr-labeled cells in the three types of cultures. c Quantification of EtBr-positive cells in mixed and neuronal retinal cultures. d Positive control showing the uptake of EtBr induced by 3 mM ATP in rat macrophage cultures. Labeled cells in each culture were counted in ten different micrographs randomly obtained. Data represent the mean ± SEM of five separate experiments performed in duplicate. ***p < 0.01 versus control. Bars = 20 μm