Fig. 3.

Autocrine effects of high glucose and palmitate on real-time luciferase cAMP production. MIN6c4 growing in 35-mm Petri dishes were transfected with Glosensor vector. After equilibrating the cells with reagent, real-time cAMP-stimulated luciferase luminescence was followed for 15 min: (a) high glucose (16.7 mmol/l) decreased cAMP levels, which was reversed by GLP-1 (1 μmol/l); (b) palmitate (100 μmol/l) inhibited cAMP production, which was reversed by GLP-1 (1 μmol/l). The shown data represent two independent experiments