Fig. 2.

Dynamin controls agonist-induced internalisation of P2X4. a Desensitisation during the ATP-induced P2X4 response was measured as the percentage decline in inward current after 4 s of ATP application. Representative normalised current is shown for P2X4-GFP alone (black trace) showing desensitisation and P2X4-GFP plus Dyn-K44A (red trace) showing minimal desensitisation. b Normal P2X4 responses declined by an average of 32.7 % which was significantly reduced in the presence of Dyn-K44A mutant to 4.1 % (**p < 0.0001; n = 9–16 cells). Error bars represent SEM