TABLE 1.
Quinolone susceptibilities and amino acid changes in GyrA and ParC QRDR fragments of the H. influenzae isolates
| Strain or no. of strains | MIC or MIC range (μg/ml)a
|
Amino acid at the following positions in QRDR of GyrA:
|
Amino acid the following positions in QRDR of ParC:
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| CIP | MOX | LEV | NAL | 83 | 84 | 88 | 82 | 83 | 84 | 88 | |
| ATCC 51907 | 0.007 | 0.03 | 0.03 | 0.5 | Asp | Ser | Asp | Gly | Asp | Ser | Glu |
| ATCC 49247 | 0.01 | 0.03 | 0.03 | 2.0 | |||||||
| 34b | 0.007-0.12 | 0.01-0.12 | 0.01-0.12 | 1.0-64 | |||||||
| 1 | 0.12 | 0.06 | 0.06 | 16 | Ala | ||||||
| 1 | 0.12 | 0.06 | 0.12 | 2.0 | Tyr | ||||||
| 1 | 0.12 | 0.12 | 0.12 | 64 | Tyr | ||||||
| 1 | 0.25 | 0.12 | 0.12 | 64 | Asn | ||||||
| 1 | 1.0 | 1.0 | 1.0 | 4.0 | Phe | ||||||
| 1 | 2.0 | 2.0 | 1.0 | 128 | Leu | ||||||
| 1 | 4.0 | 4.0 | 2.0 | 2.0 | Phe | ||||||
| 1 | 0.5 | 0.5 | 0.5 | 32 | Asn | Lys | |||||
| 1 | 0.5 | 0.5 | 0.25 | 128 | Asn | Arg | |||||
| 1 | 2.0 | 2.0 | 1.0 | 64 | Tyr | Asp | |||||
| 1 | 2.0 | 1.0 | 2.0 | 64 | Leu | Ile | |||||
| 1 | 2.0 | 1.0 | 1.0 | 128 | Phe | Arg | |||||
| 1 | 4.0 | 1.0 | 2.0 | 64 | Asn | Ile | |||||
| 1 | 4.0 | 2.0 | 2.0 | 64 | Asn | Ile | |||||
| 1 | 4.0 | 2.0 | 2.0 | 64 | Asn | Ile | |||||
| 1 | 4.0 | 2.0 | 2.0 | 128 | Asn | Ile | |||||
| 1 | 4.0 | 4.0 | 2.0 | 128 | Asn | Ile | |||||
| 1 | 4.0 | 4.0 | 2.0 | 128 | Leu | Ile | |||||
| 1 | 4.0 | 4.0 | 4.0 | 128 | Leu | Ile | |||||
| 1 | 4.0 | 2.0 | 2.0 | 64 | Phe | Lys | |||||
| 1 | 1.0 | 1.0 | 0.5 | 64 | Gly | Asp | Arg | ||||
| 1 | 4.0 | 2.0 | 2.0 | 64 | Leu | Asn | Ile | ||||
| 1 | 4.0 | 2.0 | 2.0 | 128 | Leu | Asn | Ile | ||||
| 1 | 4.0 | 2.0 | 2.0 | 128 | Leu | Asn | Ile | ||||
| 1 | 8.0 | 4.0 | 4.0 | 128 | Ile | Ala | Gly | ||||
| 1 | 16 | 8.0 | 16 | 64 | Leu | Asn | Ile | ||||
| 1 | 16 | 2.0 | 1.0 | 64 | Leu | Asn | Asn | ||||
| 1 | 32 | 32 | 32 | 2.0 | Leu | Tyr | Asp | ||||
a
CIP, ciprofloxacin; LEV, levofloxacin; MOX, moxifloxacin; NAL, nalidixic acid.
b
Additional amino acid changes were detected in the QRDR of GyrB in two strains for which the nalidixic acid MIC was 32 μg/ml: Asp489 to Asn and Thr472 to Ile.