Table 3.

Summary of all six mTOR related biomarkers expression (mean H-score) in the categorized pathological parameters.

pT: pathological stage; Pten: phosphatase and tensine homologue; p27: p27^kip; phos Akt: phoporylated Akt; phos S6: phosphorylated S6 protein; 4 EBP-1: eukaryotic translation initiation factor 4E-binding protein-1. NS: not significant.

Marker	Benign vs malignant urothelium	Mean H-score	TMA spot histology	Mean H-score	UrCa Histology	Mean H-score	pT	Mean H-score	TNM stage group	Mean H-score
Pten*	Benign	100	Non-invasive	148	Pure UrCa	74	pT0	195	0a/0is	195
							pT1	73	I	61
	Malignant (p=0.0000)	42	Invasive (p=0.0004)	61	Aggressive histology (p=0.009)	34	pT2	79	II	80
							pT3/4	47	III/IV	52
phos Akt	Benign	17	Non-invasive	12	Pure UrCa	7	pT0	23	0a/0is	23
							pT1	7	I	8
	Malignant (p=0.0000)	7	Invasive (p=0.03)	6	Aggressive histology (p=0.04)	3	pT2	6	II	7
							pT3/4	6	III/IV	5
phos S6	Benign	117	Non-invasive	43	Pure UrCa	28	pT0	25	0a/0is	25
							pT1	84	I	75
	Malignant (p=0.0000)	27	Invasive (p=0.1)	25	Aggressive histology (p=NS)	10	pT2	19	II	22
							pT3/4	19	III/IV	21
4E-BP1	Benign	9	Non-invasive	8	Pure UrCa	65	pT0	10	0a/0is	10
							pT1	39	I	42
	Malignant (p=0.0000)	32	Invasive (p=0.0055)	37	Aggressive histology (p=0.004)	29	pT2	25	II	27
							pT3/4	37	III/IV	33
c-Myc	Benign	36	Non-invasive	24	Pure UrCa	2	pT0	5	0a/0is	5
							pT1	14	I	14
	Malignant (p=0.0000)	7	Invasive (p= 0.002)	5	Aggressive histology (p=NS)	8	pT2	7	II	9
							pT3/4	6	III/IV	6
p27	Benign	69	Non-invasive	76	Pure UrCa	53	pT0	63	0a/0is	63
							pT1	93	I	97
	Malignant (p=0.0029)	51	Invasive (p= NS)	47	Aggressive histology (p=0.04)	24	pT2	58	II	65
							pT3/4	36	III/IV	36