TABLE 2.
Reactivities of the produced MAbs against related herpesvirusesd
| Immunization virus | MAb designation | Isotypeb | Specificityc
|
||||
|---|---|---|---|---|---|---|---|
| BoHV-1 | BoHV-5 | CpHV-1 | CvHV-1 | CvHV-2 | |||
| BoHV-1 | G12H3D10 | IgG1 | + | + | − | − | − |
| G13G1D3B10a | IgG2a | + | − | − | − | − | |
| G14G11F5 | IgG2b | + | − | − | − | − | |
| G16B6G10 | IgG2a | + | + | + | + | + | |
| G16F3A11 | IgG2a | + | + | − | + | − | |
| G17D8E9 | IgG1 | + | + | − | − | − | |
| G1G7G8 | IgG2a | + | + | − | + | + | |
| G3D7E8D12 | IgG1 | + | + | − | − | − | |
| G5C12F2 | IgG1 | + | + | − | + | + | |
| G6B7G2 | IgG1 | + | − | − | +/− | − | |
| G6B9E7 | IgG1 | + | + | − | − | − | |
| G6D7G2 | IgG1 | + | − | − | +/− | − | |
| G6G5G2 | IgG2a | + | + | + | + | − | |
| G6H4D6 | IgG2a | + | + | + | + | + | |
| BoHV-5 | 2D2 | ND | + | + | − | − | − |
| 2D6 | IgM | − | + | − | − | − | |
| 2H7 | IgG1 | − | + | − | − | − | |
| 3D10 | IgG1 | + | + | + | + | + | |
| 4E10 | NT | + | + | − | − | − | |
| 5D6 | NT | + | + | − | − | + | |
| CpHV-1 | 2E5E1 | IgG2a | − | − | + | − | − |
| 2E5G5G1 | IgG2a | − | − | + | − | − | |
| 3A4D9 | IgG2a | − | − | + | − | − | |
| 3A4C8G8 | IgG2a | − | − | + | − | + | |
| 4G2H2 | IgG2a | − | − | + | − | − | |
| 4G2G5G1 | IgG2a | − | − | + | − | − | |
| 5F11G10 | IgG2a | − | − | + | − | − | |
| 5F11G7D8 | IgG2a | − | − | + | − | − | |
| CvHV-1 | 1B7 | IgG1 | + | + | + | + | + |
| 5D3 | ND | + | + | + | + | + | |
| 6B9 | ND | − | + | − | + | − | |
| 6C2 | IgG3 | − | − | − | + | − | |
| 6C3 | ND | − | − | − | + | − | |
| CvHV-2 | 2A6 | IgG1 | − | − | − | − | + |
| 3A8 | NT | + | − | +/− | +/− | + | |
| 3F3 | NT | +/− | − | − | − | + | |
| 4B11 | ND | − | − | +/− | − | + | |
| 4G5 | NT | +/− | − | − | − | + | |
| 5B12 | NT | − | − | − | +/− | + | |
| 5D9 | IgG1 | − | − | − | − | + | |
| 5G10 | IgG1 | − | − | − | + | + | |
a
Boldface type indicates MAbs used in the next experiments.
b
Abbreviations: ND, not determined; NT, not tested.
c
Symbols: +, positive signal; −, negative signal; +/−, weak signal.
d
BoHV-1 MAb reactivity was tested by FACS analysis, and other MAb reactivities were tested by ELISA.