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. Author manuscript; available in PMC: 2013 Jun 21.
Published in final edited form as: Science. 2012 Dec 21;338(6114):1593–1599. doi: 10.1126/science.1228186

Figure 2. Exonic features associated with evolutionary change in alternative splicing.

Figure 2

A) Above: PSI values for eef1d exon 3 across tissues and species analyzed. Below: Eef1d gene expression values. (Mean ± SD of 3 biological replicates). PSI values were calculated only for tissues with FPKM ≥ 5. Inset: exon structure of 5' end of eef1d gene (ENSMUSG00000055762).

B) Below: Number of internal exons binned by the age of the inferred alternative splicing based on occurrence in ≥ 2 individuals. Above: The fraction of exons with length divisible by 3 and the mean and SEM of the tissue-specificity.

C) Top: mean ± SEM of PSI values of exons binned by the phylogenetic extent of alternative splicing as in (B). Middle: mean ± SEM of 3' splice site scores of exons in each bin. Bottom: mean ± SEM of 5' splice site scores. Splice sites were scored using the MaxEnt model (31). *Indicates t-test p-value < 0.05. **Indicates P < 0.001.

D) Fraction of regulatory 5mers in the downstream intron that differed between mouse and rat in exons binned by the phylogenetic extent of alternative splicing as in (B) (Mean ± SEM). *Indicates t-test P < 0.05 (t-test). **P < 0.001.