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. Author manuscript; available in PMC: 2014 Jan 15.
Published in final edited form as: Anal Chem. 2012 Dec 20;85(2):971–977. doi: 10.1021/ac302690w

Figure 4.

Figure 4

Surface plasmon resonance detection of SAPE-biotin binding. (a) Normalized transmission spectra as recorded from an array of 200 nm nanopores. The transmission peak at approximately 800 nm corresponds to the gold-water interface and was used to measure the shift in transmission. (Inset) SEM image of nanopores (diameter: 200 nm, periodicity: 500 nm) used for spectral measurements. Scale bar is 1.5 µm. Four arrays were milled on the suspended nitride area for measuring the transmission spectra, two large (16 µm × 16 µm) and two smaller (8 µm × 8 µm). (b) A graph showing shift of transmission spectra through the nanopore arrays upon SAPE binding as a function of SAPE concentration.