Table 3.

Specific expression of IFN-γ in B7-H1⁺ melanomas. IFN-γ was detected by qRT-PCR as described in Materials and Methods. These results are representative of two separate experiments. UD, undetectable (C_T greater than 45 cycles); NA, not applicable.

Patient number	Tissue source	CT (mean)*	SEM†	CT (mean)	SEM
B7-H1− tumors
1	Lymph node metastasis	UD‡	NA	33.19	0.45
2	Small bowel metastasis	UD§	NA	34.46	0.38
3	Lymph node metastasis	UD	NA	32.93	0.11
4	Lymph node metastasis	UD	NA	35.53	0.28
5	Cutaneous primary lesion	UD	NA	36.11	0.13
B7-H1+ tumors
6	Lymph node metastasis	34.67	0.49	32.41	0.16
7	Lymph node metastasis	35.00	0.32	33.55	0.36
8	Lung metastasis	33.15	0.30	31.91	0.78
9	Lung metastasis	31.80	0.07	28.43	0.02
10	Lymph node metastasis	35.17	0.22	32.51	0.22
Controls
NA	PBMCs	33.12	0.35	28.55	0.66
NA	Activated CD3+ T cells	23.60	0.08	28.33	0.11
NA	1359-mel	UD	NA	UD	NA

^*Lower values reflect greater expression.

^†SEM of triplicate samples.

^‡One of three triplicate reactions for patient 1 tumor had C_T = 41.7, and two of three were undetectable and average undetectable.

^§One of three triplicate reactions for patient 2 tumor had C_T = 43.1, and two of three were undetectable and average undetectable.