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. Author manuscript; available in PMC: 2013 Nov 27.

Published in final edited form as: Biochemistry. 2012 Nov 14;51(47):9488–9500. doi: 10.1021/bi301069r

Tandem mass spectra for two cross-linked peptide precursor ions identified from apo b5-CYP3A4 complex. Fragment ions with superscript “x” represent the cross-linked fragment ions. Ions marked with subscript α are from cyt b5, and ions with subscript β are from CYP3A4. Identified cross-linked residue pairs are linked by bold black lines. A. Ion [M+H]⁴⁺=757.3763 for apo b5: ³⁵FLEEHPGGEEVLR⁴⁷-CYP3A4: ¹¹⁶SAISIAEDEEWKR¹²⁸. B. Ion [M+H]⁵⁺=1024.8989 for apo b5: ⁴⁸EQAGGDATENFEDVGHSTDAR⁶⁸-CYP3A4:⁷¹VWGFYDGQQPVLAITDPDMIKTVLVK⁹⁶. The measurement errors for the two ions are 1.3 and 0.8 ppm, respectively.

Tandem mass spectra for two cross-linked peptide precursor ions identified from apo b5-CYP3A4 complex. Fragment ions with superscript “x” represent the cross-linked fragment ions. Ions marked with subscript α are from cyt b5, and ions with subscript β are from CYP3A4. Identified cross-linked residue pairs are linked by bold black lines. A. Ion [M+H]⁴⁺=757.3763 for apo b5: ³⁵FLEEHPGGEEVLR⁴⁷-CYP3A4: ¹¹⁶SAISIAEDEEWKR¹²⁸. B. Ion [M+H]⁵⁺=1024.8989 for apo b5: ⁴⁸EQAGGDATENFEDVGHSTDAR⁶⁸-CYP3A4:⁷¹VWGFYDGQQPVLAITDPDMIKTVLVK⁹⁶. The measurement errors for the two ions are 1.3 and 0.8 ppm, respectively.