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Titration of cyt b5 to wild-type CYP3A4 and mutations and apo b5 to wild-type CYP3A4. The spectral titration experiment was carried out using 200 nM CYP3A4/mutations and titration with cyt b5 or apo b5. The magnitude of the absorbance change at 390 minus 418 nm was measured following each addition of b5 at room temperature.
