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Effect of cyt b5/CYP ratio on formation of 6β-hydroxytestosterone by CYP3A4 and its mutations. Reactions were carried out using varying concentrations of cyt b5 and constant ratio of CYP3A4 (40 pmol) /CPR (80 pmol) for 10 min in 200 uL reaction volume. Each point represents the mean ± S.D. (n=3).
