Fig 6.

Fluorescent confocal microscopy of mammary epithelial cells during bacterial infections. SYTO 9 (A to E) and phalloidin (F to J) stainings were used to observe bMEC structure following internalization assays with S. aureus RF122 (carrying plasmid pCtuf-gfp in the case of phalloidin staining) at an MOI of 100:1. MAC-T cells were either untreated (control; A, F, and I) or treated with S. aureus alone (B, E, G, and J), L. casei CIRM-BIA 667 alone at an ROI of 2,000:1 (D), or S. aureus and L. casei in cocultures (C and H). A lens with a ×100 magnification was used, and panels D to E and I to J are electronically zoomed. Arrows indicate internalized L. casei (D) or internalized S. aureus (E and J). Cyt., cytoplasm; N., nucleus.