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RDase expression in a culture of the BAV1 strain grown on cis-DCE. Shown are the results from BN-PAGE indicating the positions of gel slices, amounts of dechlorination product(s) obtained with the different gel slices in activity tests, an SDS-polyacrylamide gel of proteins in slice 10, and the mass spectrometric identification of the band at ∼50 kDa. A positive control (+) for dechlorination was assayed with 10 μl crude protein extract, instead of protein from a gel slice.
